Details

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S-Adenosyl-L-Homocysteine Hydrolase，SAHH

Homocysteine ( Hcy )is a thiol-containing amino acid formed from methionine duringS-adenosylmethionine-dependent transmethylation reactions . It has beendemonstrated that even mild or moderately elevated levels of Hcy also increasethe risk of atherosclerosis of the coronary , cerebral and peripheral arteriesand cardiovascular disease . And currently the hcy level is regarded as thebiomarker for cardiovascular disease diagnosis all over the world .

S-adenosyl-L-homocysteinehydrolase (SAHH , EC 3.3.1.1) (AdoHcyase) is an enzyme of the activated methyl cycle, responsiblefor the reversible hydration of S-adenosyl-L-homocysteine into adenosine andhomocysteine.

SAHHis a ubiquitous enzyme which binds and requires NAD+ as a cofactor.AdoHcyase is a highly conserved protein of about 430 to 470 amino acids. Thefamily contains a glycine-rich region in the central part of AdoHcyase; aregion thought to be involved in NAD-binding. This protein may use themorpheein model of allosteric regulation.

There are severalmethods for hcy level detection . And in all these methods the most time-saving, high sensitivity , high throughput is enzyme cycling methods . People hasapplied this method for hcy diagnosis kit , and this kit is very popularthroughout the world .

The principle of the enzyme cycling methods is as the following :

Hcy: Homocysteine SAM: S-adenosyl-L-methionine HMT: Homocysteine

methyltransferase SAH: S-adenosyl-L-homocysteine SAHH:S-adenosyl-L-homocysteine

hydrolase ADA: Adenosine Deaminase

The SAHH we can provide is recombinant protein , and it is of highactivity and good stability, which can be used for different applications .

Product description:

Appearance:White powder, lyophilized

Preservationsolution: Tris buffer, pH8.0

Specificactivity: 10U/mg

BUnit Definition:One unit will catalyze 1.0 μmoleof S-adenosyl-L-homocysteine to adenosine and Hcy per min at pH 7.4 at37°C.

Basic information:

Cas #: LM-130

Source: Recombinantexpression

pH Stability: pH6.0-8.0 pI: 6.15

Molecular weight: About44kDa (SDS-PAGE detection)

Purity: ≥90%(SDS-PAGE test)

Redissolution and storage: Redissolvedin 20% glycerol, 4℃,store at -20℃ for long-termpreservation, Avoid multiple freeze-thaw cycles.

Transportation: Coldpacks

Security:Only used for research, strictly prohibited from human or animal experiment.