Details

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Superoxide Dismutase，Mn-SOD

Superoxide Dismutase（SOD，EC1.15.1.1)deals with the superoxide radical by alternately adding or removing an electronfrom the superoxide molecules it encounters. Based on the metalion cofactoridentified in their active site, four types of SODs (Cu/Zn-SOD, Fe-SOD,Mn-SOD and Ni- SOD) are reported. Mn-SOD is presented in the mitochondria ofeukaryotic cells and prokaryotic cells. The animal-free Mn-SOD with higherpurity and activity is expressed by E. coli.

BiochemicalResearch: SOD is a unique enzyme which caneliminate superoxide radical, thus protecting the cell from superoxidetoxicity. SOD is widely used for adjusting endocrine system and immunityenhancement, in clinical and research of inflammation, such as therapy rheumatoidarthritis, Multiple chronic arthritis, myocardial infarction, angiocardiopathy,cancer patients.

Productdescription:

Appearance:Whitepowder, lyophilized

Preservation solution: 100mMphosphate buffer，pH7.4

Specific Activity: 15,528U/mg protein.

Unit Definition: pH8.2,54 mM Tris-Cl 140 uL, including 54 mM Dimethyl swollen acid sodium, 1.07 mMdiethylenetriamine pentaacetic acid, 5 uLddH2O or ( 5uL pyrogallicacid in10 mM HCI); total reaction volume is 150 uL, timekeeping. Theautoxidation rate is effective within 3 minutes，controllingthe quantity of pyrogallic acid, keeping the autoxidation rate will produce aincrease per min by 0.018 at 420 nm min，andproduce a increase per min by 0.010 after SOD adding.

Basic information:

Cas#: LM-126

Source: Recombinantexpression

Molecular weight: About26kDa (SDS-PAGE detection)

PPurity: ≥90%(SDS-PAGE test)

Storage: 4℃,store at -20℃for long-term preservation

Transportation:Cold packs

Safety tips: Donot use for human experiments