Limin International Group Co. Ltd ，a fast-growing company founded by returned scholars and their partners, is mainly engaged in R&D, production, and sales of bioactive proteins and clinical diagnostis reagents
Category：Recombinant Proteins
Description：UricaseTheenzymeuricase(EC1.7.3.3),orurateoxidase(UO) or factor-independenturatehydroxylase,
absentinhumans,catalyzestheoxidationofuricacidto5-hydroxyisourate:Urateoxidaseismainlylocalisedintheliver
Details
Uricase
The enzyme uricase (EC1.7.3.3), or urate oxidase(UO) or factor-independent urate hydroxylase, absent in humans,catalyzes theoxidation of uric acidto 5-hydroxyisourate :
Uric acid +o2+ h20-5-hydroxyisourate +H202 -allantoin + Co2 Uricase is slightly dissolved in alkaline buffer, PH6.3 and the enzyme activi inhibited by cyanide, Ag and Hg2+
Urate oxidase is mainly localised in theliver, where it forms a large electron-dense paracrystalline core in many peroxisomes. Theenzyme exists as a tetramer of identical subunits, each containing a possibletype 2 copper-binding site.
Urate oxidase is found in nearly allorganisms, from bacteria to mammals , and plays different metabolic roles,depending on its host organism. It was lost in early primateevolution, and sois absent in humans and other higher apes.
Theuric acid is hard to be dissolved and the high concentration of uric acid willdeposit in the organization to form a stone. When uricase is used for treatmentof hyperuricaemia patients, it can reduce the levels of uric acid in the blood,increase allantoate excretion and then eliminate the uric acid strand in theblood and tissues. It is also used to detect the amount of uric acid in theserum and urine.
Theuricase provided by our company is recombinant protein. It is of high purityand good activity. The use of recombinant uricase eliminates the risk of viruspresence, and other potential adventitious agents found in animal-deriveduricase.
Biochemicalstudies: Uricase cancatalyze the enzymatic oxidation of uric acid to allantoate rapidly, so it isused to detect the amount of uric acid in the serum and urine.
Productdescription:
Appearance:White powder, lyophilized
Preservationsolution: 20mM Tris, pH8.0
Specificactivity: About 10U/mg
Unit definition: Oneunit will convert 1μmoluric acid to oxidize per min at pH 8.5 and at 37℃.
Basic information:
Cas #: LM-135
Source: Recombinantexpression
Molecular weight: About45kDa (SDS-PAGE detection)
Purity: ≥90%(SDS-PAGE test)
Storage: 4℃,store at -20℃ for long-termpreservation
Transportation:Cold packs
Safety tips: Donot use for human experiments