Limin International Group Co. Ltd ，a fast-growing company founded by returned scholars and their partners, is mainly engaged in R&D, production, and sales of bioactive proteins and clinical diagnostic reagents.
3α-Hydroxysteroid Dehydrogenase (HSD, EC 126.96.36.199) , consisting of 258 amino acid residues with the molecular weight about 26.4kDa, is a steroid dehydrogenase secreted by Comamonas testosterone..3α-hydroxysteroid dehydrogenase can use severalkinds of steroid matrix as its substrates, which can reversible catalyze oxidationand reduction reaction of C19-27 steroid3 hydroxy
ketone radical. Theisoelectricpoint of HSD is 4.8,and its most suitable pH is 7-9. Hg2+,Ag+ can inhibit the activity of the enzyme. Metal ion chelatingagent such as EDTA will not inhibit the enzyme activity, On the contrary, it will help the enzyme to keep stability to a certain extent .
The HSD provided by our company isrecombinant protein. It is of high purity, good activity.
Biochemicalstudies: bile acid is one of the substrates of 3α-hydroxy steroiddehydrogenase. 3α-hydroxy steroid dehydrogenase is used to catalyzethe dehydrogenation reaction of hydroxy steroid inclinic. So, HSD is used to detect the total bile acid clinically.
Product details：Appearance: White powder, lyophilized Preservation solution:20mM Tris, pH8.0
Specific activity: About 50U/mg powder Unit definition: One unit will catalyze the oxidation of 1μmol of androsteroneper min at pH8.9 at 25℃.
Basic information:Cas#: LM-122
Source: Recombinant expression
Molecular weight: About 28 kDa (SDS-PAGE detection)
Purity: 90% (SDS-PAGE test)
Storage: 4℃, store at -20℃ for long-term preservation
Transportation: Cold packs
Safety tips: Do not usefor human experiments