Details

---

RecombinantPig Carboxypeptidase B （ CPB ）

Carboxypeptidase B (EC 3.4.17.2),also well known as protaminase, pancreatic procarboxy-peptidase B(PCPB), tissuecarboxypeptidase B, peptidyl-L-lysine (L-arginine) hydrolaseis a highly pancreas-specific protein (PASP), and has beenidentified previously as a serum marker for acute pancreatitis and pancreaticgraft rejection. As the prototype for those exopeptidases that cleave off basicC-terminal residues, CPB1specifically cleaves the C-terminal Arg and Lysresidues with a preference for Arg. The B1 and B2 forms of procarboxypeptidaseB differ from each other mainly in isoelectric point.The deduced amino acidsequence of CPB predicts a 416-amino acid preproenzyme consisting of a 15-aasignal peptide, a 95-aa activation peptide and a 307-aa mature chain.Thesecreted CPB zymogen is converted to enzymatically active CPB by limited proteolysisby trypsin.

During the industrial production ofinsulin, the CPB is necessary.

The CPB we provide is recombinantprotein , and it is of high activity and stability, which can be used fordifferent applications .

Productdescription:

Appearance: White powder, lyophilized

Preservationsolution: 20mM NaAc, pH3.5

Specificactivity: ≥180U/mg

Unit Definition:One unit will catalyze 1.0 μmoleof Hip-L-Arg per min at pH 7.65 at 25°C.

Basic information:

Cas#: LM-110

Source: Recombinantexpression

Storage/Stability：

Molecular weight: About35kDa(SDS-PAGE detection)

Purity: ≥90%(SDS-PAGE test)

Redissolution and storage: Redissolvedin 20% glycerol, 4℃,store at -20℃ for long-termpreservation, Avoid multiple freeze-thaw cycles.

Transportation: Coldpacks

Security:Only used for research, strictly prohibited from human or animal experiment.