Details

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Recombinant Bovine Trypsin，BT

Trypsin(EC3.4.21.4) isaserine proteasefrom thePA clansuperfamily, found in thedigestive systemof many vertebrates, where ithydrolysesproteins. Trypsin is produced in thepancreasas the inactiveproenzymetrypsinogen. Trypsin cleavespeptidechains mainly at thecarboxylside of theamino acids lysineorarginine,except when either is followed byproline. It is used for numerousbiotechnologicalprocesses. The process is commonlyreferred to as trypsinproteolysisortrypsinisation, nd proteins that have been digested/treated withtrypsin are said to have been trypsinized.

Trypsin is available in high quantity inpancreases, and can be purified rather easily. Hence it has been used widely invarious biotechnological processes.

Trypsin can be used to re-suspend cellsadherent to the cell culture dish wall during the process of harvesting cells.Trypsin can also be used to dissociate dissected cells (for example,prior to cell fixing and sorting).

Trypsin is commonly used in biologicalresearch duringproteomicsexperiments to digest proteins into peptides for mass spectrometry analysis,e.g.in-gel digestion. Trypsin isparticularly suited for this, since it has a very well defined specificity, asit hydrolyzes only the peptide bonds in which the carbonyl group is contributedeither by an Arg or Lys residue. Trypsin can also be used to dissolve bloodclots in its microbial form and treat inflammation in its pancreatic form.

During the industrial production of insulin , bovine trypsin is necessary. Especially forinsulin analog , the bovine trypsin isof extreme importance ,since other kinds of trypsins ( such as those from human, mouse ,and so on ) can not work insulin analog well .

The trypsin we can provide isrecombinant proteins , and it is of high activity and stability, which can beused for different applications . The use of recombinant bovine Trypsineliminates the risk of virus presence, and other potential adventitious agentsfound in animal-derived trypsin.

Productdescription:

Appearance: Colorlessaqueous solution

Formulation:Bovine Trypsin is supplied in 20mM NaAc，pH4.5

BiologicalActivity: 150Units/mg protein

UnitDefinition: One unit will producea increaseby 0.18 per min at 247nm, at pH8.1, at 25°C, using TAME assubstrate, Reaction volume = 3.0 mL (1 cm light path).

General Description:

Cas#: LM-133

Source: Recombinantexpression

EC number: EC3.4.21.4

Molecular Mass: 25KDa（Determinedby SDS-PAGE）

Purity: ≥90%（Determinedby SDS-PAGE）

Storage: 4℃,store at -20℃/-80℃ for long-term preservation，Avoidmultiple freeze-thaw cycles.

Transportation:Cold packs

Safety tips: Strictlyprohibited from human experiments

Usage：Usingfor product of recombinant insulin.